Acetylated lysine polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Acetylated lysine polyclonal antibody (Cat # PAB10348) is shown to detect Acetylated lysine in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).
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Specification
Product Description
Rabbit polyclonal antibody raised against Acetylated lysine.
Immunogen
Acetylated lysine conjugated with KLH.
Host
Rabbit
Reactivity
Mammals
Specificity
Reactivity is specific to acetylated lysine.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Acetylated Lysine.
Recommend Usage
ELISA (1:2500)
Western Blot (1:1000)
Immunoprecipitation (10 ug/mg sample)
Immunofluorescence (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Acetylated lysine polyclonal antibody (Cat # PAB10348) is shown to detect Acetylated lysine in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Acetylation dependent functions of Rab22a-NeoF1 Fusion Protein in Osteosarcoma.
Xiaoting Liang, Xin Wang, Yaohui He, Yuanzhong Wu, Li Zhong, Wen Liu, Dan Liao, Tiebang Kang.
Theranostics 2020 Jun; 10(17):7747.
Application：WB-Tr, Human, HEK 293T cells.
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Acetylation-dependent function of human single-stranded DNA binding protein 1.
Wu Y, Chen H, Lu J, Zhang M, Zhang R, Duan T, Wang X, Huang J, Kang T.
Nucleic Acids Research 2015 Sep; 43(16):7878.
Application：IP-WB, Human, HEK 293T cells.
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Acetylation dependent functions of Rab22a-NeoF1 Fusion Protein in Osteosarcoma.
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