PDPK1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using PDPK1 polyclonal antibody (Cat # PAB10309) shows detection of myc-tagged human PDPK1 at 60 KDa in ~10 ug of a virus infected Sf9 cell lysate (arrow).
The nitrocellulose membrane was probed overnight at 4°C with the primary antibody diluted 1 : 750 in 1% non-fat dry milk.
HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDPK1.
Immunogen
A synthetic peptide corresponding to C-terminus of human PDPK1.
Host
Rabbit
Reactivity
Dog, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against human PDK-1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:100000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot using PDPK1 polyclonal antibody (Cat # PAB10309) shows detection of myc-tagged human PDPK1 at 60 KDa in ~10 ug of a virus infected Sf9 cell lysate (arrow).
The nitrocellulose membrane was probed overnight at 4°C with the primary antibody diluted 1 : 750 in 1% non-fat dry milk.
HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PDPK1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B.
Scheid MP, Marignani PA, Woodgett JR.
Molecular and Cellular Biology 2002 Sep; 22(17):6247.
Application：IF, WB-Tr, Human, HEK 293 cells.
-
High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site.
Biondi RM, Komander D, Thomas CC, Lizcano JM, Deak M, Alessi DR, van Aalten DM.
The EMBO Journal 2002 Aug; 21(16):4219.
-
Involvement of Hsp90 in signaling and stability of 3-phosphoinositide-dependent kinase-1.
Fujita N, Sato S, Ishida A, Tsuruo T.
The Journal of Biological Chemistry 2002 Mar; 277(12):10346.
Application：IP, WB, Human, HEK 293T, HT1080, NL-17, PC-3 cells.
-
Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity.
Park J, Hill MM, Hess D, Brazil DP, Hofsteenge J, Hemmings BA.
The Journal of Biological Chemistry 2001 Oct; 276(40):37459.
-
Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com