PACRG polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using PACRG polyclonal antibody (Cat # PAB10304) shows detection of aband ~ 40 KDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this bandis blocked when the antibody is pre-incubated withthe immunizing peptide (Lane 2).
Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PACRG.
Immunogen
A synthetic peptide corresponding to amino acids 204-215 of human PACRG.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against human PACRG protein. Expect reactivity with both splice variants of this protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PACRG polyclonal antibody (Cat # PAB10304) shows detection of aband ~ 40 KDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this bandis blocked when the antibody is pre-incubated withthe immunizing peptide (Lane 2).
Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PACRG
Entrez GeneID
135138Protein Accession#
Q96M98;AAH30642Gene Name
PACRG
Gene Alias
FLJ32724, GLUP, HAK005771, PARK2CRG, RP3-495O10.2
Gene Description
PARK2 co-regulated
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017729|OTTHUMP00000017730|molecular chaperone/chaperonin-binding protein|parkin co-regulated gene protein
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Interactome
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Disease
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Publication Reference
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Susceptibility to leprosy is associated with PARK2 and PACRG.
Mira MT, Alcaïs A, Nguyen VT, Moraes MO, Di Flumeri C, Vu HT, Mai CP, Nguyen TH, Nguyen NB, Pham XK, Sarno EN, Alter A, Montpetit A, Moraes ME, Moraes JR, Doré C, Gallant CJ, Lepage P, Verner A, Van De Vosse E, Hudson TJ, Abel L, Schurr E.
Nature 2004 Feb; 427(6975):636.
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A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death.
Imai Y, Soda M, Murakami T, Shoji M, Abe K, Takahashi R.
The Journal of Biological Chemistry 2003 Dec; 278(51):51901.
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Identification of a novel gene linked to parkin via a bi-directional promoter.
West AB, Lockhart PJ, O'Farell C, Farrer MJ.
Journal of Molecular Biology 2003 Feb; 326(1):11.
Application：WB, Human, Mouse, Brains, SH-SY5Y cells.
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Susceptibility to leprosy is associated with PARK2 and PACRG.
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