MRE11 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
ChIP
Chromatin Immunoprecipitation (ChIP) using MRE11 polyclonal antibody (Cat # PAB10277).
A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes).
After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions.
Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies.
The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus).
Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MRE11.
Immunogen
A synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae MRE11.
Host
Rabbit
Reactivity
Yeast
Specificity
This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
ChIP
Chromatin Immunoprecipitation (ChIP) using MRE11 polyclonal antibody (Cat # PAB10277).
A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes).
After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions.
Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies.
The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus).
Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.Western Blot
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MRE11
Entrez GeneID
855264Protein Accession#
P32829;NP_013951Gene Name
MRE11
Gene Alias
NGS1, RAD58, XRS4
Gene Description
Mre11p
Gene Ontology
HyperlinkGene Summary
exhibits nuclease activity that appears to be required for RMX function; widely conserved
Other Designations
Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved
-
Publication Reference
-
The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks.
Paull TT, Gellert M.
Molecular Cell 1998 Jun; 1(7):969.
-
Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae.
Johzuka K, Ogawa H.
Genetics 1995 Apr; 139(4):1521.
Application：WB-Ce, WB-Tr, Yeast, Yeast cells.
-
Functions of the yeast meiotic recombination genes, MRE11 and MRE2.
Ogawa H, Johzuka K, Nakagawa T, Leem SH, Hagihara AH.
Advances in Biophysics 1995 Jan; 31:67.
Application：WB-Re, WB-Tr, Yeast, Recombinant proteins, Yeast cells.
-
The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com