Ldb2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using Ldb2 polyclonal antibody (Cat # PAB10273) showsdetection of a 43 KDa band corresponding to Ldb2 in a lysates prepared from human kidney (Lane 1) and mouse spleen (Lane 2) tissues.
Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Ldb2 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hourat room temperature.
A chemiluminescence system was used for signal detection using a 1 min exposure time.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with Ldb2 polyclonal antibody (Cat # PAB10273) was used at a 5 ug/mL to detect Ldb2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ldb2.
Immunogen
A synthetic peptide corresponding to amino acids 107-120 of mouse Ldb2.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse LDB2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Ldb2 polyclonal antibody (Cat # PAB10273) showsdetection of a 43 KDa band corresponding to Ldb2 in a lysates prepared from human kidney (Lane 1) and mouse spleen (Lane 2) tissues.
Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Ldb2 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hourat room temperature.
A chemiluminescence system was used for signal detection using a 1 min exposure time.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with Ldb2 polyclonal antibody (Cat # PAB10273) was used at a 5 ug/mL to detect Ldb2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ldb2
Entrez GeneID
16826Protein Accession#
O55203;NP_034828Gene Name
Ldb2
Gene Alias
AI035351, AW146358, CLIM1, CLP-36, Ldb3
Gene Description
LIM domain binding 2
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Ubiquitination-dependent cofactor exchange on LIM homeodomain transcription factors.
Ostendorff HP, Peirano RI, Peters MA, Schluter A, Bossenz M, Scheffner M, Bach I.
Nature 2002 Mar; 416(6876):99.
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Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis.
Toyama R, Kobayashi M, Tomita T, Dawid IB.
Mechanisms of Development 1998 Feb; 71(1-2):197.
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A family of LIM domain-associated cofactors confer transcriptional synergism between LIM and Otx homeodomain proteins.
Bach I, Carriere C, Ostendorff HP, Andersen B, Rosenfeld MG.
Genes & Development 1997 Jun; 11(11):1370.
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Ubiquitination-dependent cofactor exchange on LIM homeodomain transcription factors.
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