Products

Western Blot (Tissue lysate)

Western blot using LIMK1 polyclonal antibody (Cat # PAB10258) shows detection of a 73 KDa band corresponding to LIMK1 in lysates from mouse brain.
Approximately 18 ug of lysate wasrun on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of LIMK1 polyclonal antibody.
The doublet band at ~ 75 KDa may represent phosphorylated and non-phosphorylated forms of the protein.
The identity of the strong lower molecular weight band at approximately 20 KDa is unknown.
Signal was detected using standard techniques.

Immunofluorescence

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

3984

Protein Accession#

P53667;NP_002305(isoform1)

Gene Name

LIMK1

Gene Alias

LIMK

Gene Description

LIM domain kinase 1

Omim ID

601329

Gene Ontology

Hyperlink

Gene Summary

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq

Other Designations

LIM motif-containing protein kinase|OTTHUMP00000025066