FBXW11 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FBXW11.
Immunogen
A synthetic peptide corresponding to N-terminus of human FBXW11.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody reacts with human betaTrCP2 protein.
Form
Liquid
Purification
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Recommend Usage
ELISA (1:10000-1:100000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C as an undiluted liquid for several weeks. For long term storage, aliquot and store at -20°C or below.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using FBXW11 polyclonal antibody (Cat # PAB10237) shows detection of mouse and human FBXW11 (arrowhead) in NIH/3T3 (Lane 1) and 293 (Lane 2) whole cell lysates, respectively.
The band appears as a 58 KDa protein, although a 62.1 KDa band is predicted.
The identity of faint higher molecular weight bands is not known.
The primary antibody was used at a 1 : 200 dilution incubated in 5% BLOTTO overnight at 4°C.
Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1 : 20,000 in blocking buffer for 1 h at 4°C.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXW11
Entrez GeneID
23291Protein Accession#
NP_387449; Q9UKB1Gene Name
FBXW11
Gene Alias
BTRC2, BTRCP2, FBW1B, FBXW1B, Fbw11, Hos, KIAA0696
Gene Description
F-box and WD repeat domain containing 11
Omim ID
605651Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons. [provided by RefSeq
Other Designations
F-box and WD-40 domain protein 11|F-box and WD-40 domain protein 1B|F-box protein Fbw1b|beta-transducin repeat-containing protein 2
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Interactome
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Pathway
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Disease
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Publication Reference
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M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP.
Watanabe N, Arai H, Nishihara Y, Taniguchi M, Watanabe N, Hunter T, Osada H.
PNAS 2004 Mar; 101(13):4419.
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A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G.
Nature Cell Biology 2004 Feb; 6(2):97.
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CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex.
Dias DC, Dolios G, Wang R, Pan ZQ.
PNAS 2002 Dec; 99(26):16601.
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M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP.
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