RNF2 polyclonal antibody
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More Files
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Western Blot
Western blot using RNF2 polyclonal antibody (Cat # PAB10232) shows detection of a 38 KDa band corresponding to human RNF2 in 3T3 (Lane 1), U937 (Lane 2), Jurkat (Lane 3), mouse brain (Lane 4) and CHO-K1 (Lane 5) cell lysates.
Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilutionof RNF2 polyclonal antibody incubated at room temperature.
Signal was detected using standard techniques.
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Specification
Product Description
goat polyclonal antibody raised against synthetic peptide of RNF2.
Immunogen
A synthetic peptide corresponding to amino acids 189-201 of human RNF2.
Host
Goat
Reactivity
Bovine, Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Orangutan, Rat
Specificity
This affinity purified antibody is directed against human RING1B protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using RNF2 polyclonal antibody (Cat # PAB10232) shows detection of a 38 KDa band corresponding to human RNF2 in 3T3 (Lane 1), U937 (Lane 2), Jurkat (Lane 3), mouse brain (Lane 4) and CHO-K1 (Lane 5) cell lysates.
Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilutionof RNF2 polyclonal antibody incubated at room temperature.
Signal was detected using standard techniques.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF2
Entrez GeneID
6045Protein Accession#
Q99496;NP_009143Gene Name
RNF2
Gene Alias
BAP-1, BAP1, DING, HIPI3, RING1B, RING2
Gene Description
ring finger protein 2
Omim ID
608985Gene Ontology
HyperlinkGene Summary
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq
Other Designations
OTTHUMP00000033405|OTTHUMP00000060668
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Interactome
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Disease
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Publication Reference
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Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition.
Voncken JW, Roelen BA, Roefs M, de Vries S, Verhoeven E, Marino S, Deschamps J, van Lohuizen M.
PNAS 2003 Mar; 100(5):2468.
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Involvement of the Polycomb-group gene Ring1B in the specification of the anterior-posterior axis in mice.
Suzuki M, Mizutani-Koseki Y, Fujimura Y, Miyagishima H, Kaneko T, Takada Y, Akasaka T, Tanzawa H, Takihara Y, Nakano M, Masumoto H, Vidal M, Isono K, Koseki H.
Development 2002 Sep; 129(18):4171.
Application：IF, IP-WB, WB-Ti, Human, Mouse, Embryos, U2OS cells.
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Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors.
Tuckfield A, Clouston DR, Wilanowski TM, Zhao LL, Cunningham JM, Jane SM.
Molecular and Cellular Biology 2002 Mar; 22(6):1936.
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Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition.
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