DIAPH2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using DIAPH2 polyclonal antibody (Cat # PAB10201) shows detection of a 132 KDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells.
Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used forsignal detection using a 1 min exposure time.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DIAPH2.
Immunogen
A synthetic peptide corresponding to amino acids 1085-1101 of human DIAPH2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:12000-1:60000)
Western Blot (1:1000-1:6000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using DIAPH2 polyclonal antibody (Cat # PAB10201) shows detection of a 132 KDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells.
Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of DIAPH2 polyclonal antibody.
Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used forsignal detection using a 1 min exposure time.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — DIAPH2
Entrez GeneID
1730Protein Accession#
O60879;NP_006720Gene Name
DIAPH2
Gene Alias
DIA, DIA2, DRF2, FLJ11167, POF, POF2
Gene Description
diaphanous homolog 2 (Drosophila)
Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000024270|OTTHUMP00000062171|diaphanous 2|diaphanous-related formin-2
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification and characterization of human FMNL1, FMNL2 and FMNL3 genes in silico.
Katoh M, Katoh M.
International Journal of Oncology 2003 May; 22(5):1161.
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A human homologue of the Drosophila melanogaster diaphanous gene is disrupted in a patient with premature ovarian failure: evidence for conserved function in oogenesis and implications for human sterility.
Bione S, Sala C, Manzini C, Arrigo G, Zuffardi O, Banfi S, Borsani G, Jonveaux P, Philippe C, Zuccotti M, Ballabio A, Toniolo D.
American Journal of Human Genetics 1998 Mar; 62(3):533.
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Nonsyndromic deafness DFNA1 associated with mutation of a human homolog of the Drosophila gene diaphanous.
Lynch ED, Lee MK, Morrow JE, Welcsh PL, Leon PE, King MC.
Science 1997 Nov; 278(5341):1315.
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Identification and characterization of human FMNL1, FMNL2 and FMNL3 genes in silico.
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