Eif2ak3 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis using Eif2ak3 polyclonal antibody (Cat # PAB10175) to detect Eif2ak3 in cell lysates. 300 ug Eif2ak3 over-expressing 293T cell lysate (lanes 1 & 2), or 800 ug wild type (Lanes 3 & 4), and Eif2ak3 knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15 uL anti-Eif2ak3, followed by immunobloting with 1:1000 dilution of Eif2ak3 polyclonal antibody in 5% milk/TBST buffer.
Lane 1, 293T cells over-expressing Myc-Eif2ak3 wt, Lane 2, 293T cells over-expressing Myc-Eif2ak3 K618A.
Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Eif2ak3 polyclonal antibody (Cat # PAB10175).
Positive staining signal observed in wild type mouse sample with Eif2ak3 polyclonal antibody staining only (B) , but not in the knock out mouse sample (C) and pre-immune serum staining (A) .
The Eif2ak3 polyclonal antibody (Cat # PAB10175) was diluted in 1 : 1,000.
Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Eif2ak3.
Immunogen
Recombinant protein corresponding to amino acids 601-1115 of mouse Eif2ak3.
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500-1:3000)
Immunoprecipitation (10-30ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using Eif2ak3 polyclonal antibody (Cat # PAB10175) to detect Eif2ak3 in cell lysates. 300 ug Eif2ak3 over-expressing 293T cell lysate (lanes 1 & 2), or 800 ug wild type (Lanes 3 & 4), and Eif2ak3 knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15 uL anti-Eif2ak3, followed by immunobloting with 1:1000 dilution of Eif2ak3 polyclonal antibody in 5% milk/TBST buffer.
Lane 1, 293T cells over-expressing Myc-Eif2ak3 wt, Lane 2, 293T cells over-expressing Myc-Eif2ak3 K618A.
Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Eif2ak3 polyclonal antibody (Cat # PAB10175).
Positive staining signal observed in wild type mouse sample with Eif2ak3 polyclonal antibody staining only (B) , but not in the knock out mouse sample (C) and pre-immune serum staining (A) .
The Eif2ak3 polyclonal antibody (Cat # PAB10175) was diluted in 1 : 1,000.
Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Eif2ak3
Entrez GeneID
13666Protein Accession#
AAH54809;Q9Z2B5Gene Name
Eif2ak3
Gene Alias
AI427929, Pek, Perk
Gene Description
eukaryotic translation initiation factor 2 alpha kinase 3
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Nrf2 as a novel molecular target for chemoprevention.
Lee JS, Surh YJ.
Cancer Letters 2005 Jun; 224(2):171.
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Nrf2 is a direct PERK substrate and effector of PERK-dependent cell survival.
Cullinan SB, Zhang D, Hannink M, Arvisais E, Kaufman RJ, Diehl JA.
Molecular and Cellular Biology 2003 Oct; 23(20):7198.
Application：IP, Mouse, NIH/3T3 cells.
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Dimerization and release of molecular chaperone inhibition facilitate activation of eukaryotic initiation factor-2 kinase in response to endoplasmic reticulum stress.
Ma K, Vattem KM, Wek RC.
The Journal of Biological Chemistry 2002 May; 277(21):18728.
Application：IP, WB-Tr, Human, HEK 293T cells.
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Nrf2 as a novel molecular target for chemoprevention.
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