Ankrd26 polyclonal antibody
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More Files
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Western Blot
Western blot using Ankrd26 polyclonal antibody (Cat # PAB10090) shows detection of a band at ~81 KDa corresponding to mouse Ankrd26 protein.
Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3MES cell lysate-40 ug, Lane 4 293T-Ankrd26 transfected cell lysate-20 ug, Lane 5 control 293T celllysate-20 ug, Lane 6 BSA-Ankrd26 conjugate-20ng, Lane 7 BSA-500 ng, Lane 8 BSA-100 ng, Lane 9 BSA-20 ng.
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used.
Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed withthe primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000.
Sizeestimation was made by comparison to prestained MW markers as indicated.
Personal Communication.Ira Pastan, NIH, CCR, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ankrd26.
Immunogen
A synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Specificity
This affinity-purified antibody is directed against mouse Ankrd26 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:80000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using Ankrd26 polyclonal antibody (Cat # PAB10090) shows detection of a band at ~81 KDa corresponding to mouse Ankrd26 protein.
Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3MES cell lysate-40 ug, Lane 4 293T-Ankrd26 transfected cell lysate-20 ug, Lane 5 control 293T celllysate-20 ug, Lane 6 BSA-Ankrd26 conjugate-20ng, Lane 7 BSA-500 ng, Lane 8 BSA-100 ng, Lane 9 BSA-20 ng.
Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used.
Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed withthe primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000.
Sizeestimation was made by comparison to prestained MW markers as indicated.
Personal Communication.Ira Pastan, NIH, CCR, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ankrd26
Entrez GeneID
232339Protein Accession#
AAH47067;Q69ZS2Gene Name
Ankrd26
Gene Alias
5730521P14Rik, BC008111, mKIAA1074
Gene Description
ankyrin repeat domain 26
Gene Ontology
HyperlinkOther Designations
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