GGA3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using GGA3 polyclonal antibody (Cat # PAB10050) shows detection of aband at ~110 KDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein (Lane 2, arrowhead).
Pre-incubation of antibody with immunizing peptide blocks specific staining (lane3).
MW markers are shown in lane 1 (700 nm channel, red).
Approximately 35 ug of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:600.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GGA3.
Immunogen
A synthetic peptide corresponding to amino acids 400-415 of human GGA3.
Host
Rabbit
Reactivity
Human, Macaque, Orangutan
Specificity
The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants for this protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot using GGA3 polyclonal antibody (Cat # PAB10050) shows detection of aband at ~110 KDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein (Lane 2, arrowhead).
Pre-incubation of antibody with immunizing peptide blocks specific staining (lane3).
MW markers are shown in lane 1 (700 nm channel, red).
Approximately 35 ug of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:600.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — GGA3
Entrez GeneID
23163Protein Accession#
Q9NZ52;NP_619525Gene Name
GGA3
Gene Alias
KIAA0154
Gene Description
golgi associated, gamma adaptin ear containing, ARF binding protein 3
Omim ID
606006Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq
Other Designations
ADP-ribosylation factor binding protein 3|Golgi-localized, gamma ear-containing, ARF-binding protein 3
-
Interactome
-
Pathway
-
Publication Reference
-
Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins.
Prag G, Lee S, Mattera R, Arighi CN, Beach BM, Bonifacino JS, Hurley JH.
PNAS 2005 Feb; 102(7):2334.
-
The GAT domains of clathrin-associated GGA proteins have two ubiquitin binding motifs.
Bilodeau PS, Winistorfer SC, Allaman MM, Surendhran K, Kearney WR, Robertson AD, Piper RC.
The Journal of Biological Chemistry 2004 Dec; 279(52):54808.
-
Structural basis for acidic-cluster-dileucine sorting-signal recognition by VHS domains.
Misra S, Puertollano R, Kato Y, Bonifacino JS, Hurley JH.
Nature 2002 Feb; 415(6874):933.
-
Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com