CHEK2 (phospho T68) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using CHEK2 (phospho T68) polyclonal antibody (Cat # PAB10034) shows detection of apredominant band at ~60 kDa corresponding to phosphorylated CHEK2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin.
CHEK2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production.
Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T68 of human CHEK2.
Host
Rabbit
Reactivity
Chimpanzee, Human
Specificity
Reactivity occurs against human CHK2 pT68 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. This antibody does not cross-react with Chk2 phosphorylated at other sites.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using CHEK2 (phospho T68) polyclonal antibody (Cat # PAB10034) shows detection of apredominant band at ~60 kDa corresponding to phosphorylated CHEK2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin.
CHEK2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production.
Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHEK2
Entrez GeneID
11200Protein Accession#
O96017Gene Name
CHEK2
Gene Alias
CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
Gene Description
CHK2 checkpoint homolog (S. pombe)
Gene Ontology
HyperlinkGene Summary
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028871|checkpoint-like protein CHK2|protein kinase CHK2|serine/threonine-protein kinase CHK2
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Interactome
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Pathway
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Disease
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Publication Reference
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A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage.
Brown AL, Lee CH, Schwarz JK, Mitiku N, Piwnica-Worms H, Chung JH.
PNAS 1999 Mar; 96(7):3745.
Application：WB-Ce, WB-Tr, Human, HEK 293, HeLa cells.
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A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase.
Blasina A, de Weyer IV, Laus MC, Luyten WH, Parker AE, McGowan CH.
Current Biology 1999 Jan; 9(1):1.
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Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
Matsuoka S, Huang M, Elledge SJ.
Science 1998 Dec; 282(5395):1893.
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A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage.
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