ECT2 (phospho T790) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using ECT2 (phospho T790) polyclonal antibody (Cat # PAB10006) showsdetection of endogenous phospho-ECT2 (arrowhead) present in cell lysates from interphase (Lane 1) and mitotic (Lane 2) HeLa cells.
Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA resultsusing both phosphorylated and non-phosphorylated immunizing peptide.
After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 1,000.
Personal Communication, Toru Miki,CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ECT2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T790 of human ECT2.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Human, Mouse, Rat, Zebra fish
Specificity
Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:1000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using ECT2 (phospho T790) polyclonal antibody (Cat # PAB10006) showsdetection of endogenous phospho-ECT2 (arrowhead) present in cell lysates from interphase (Lane 1) and mitotic (Lane 2) HeLa cells.
Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA resultsusing both phosphorylated and non-phosphorylated immunizing peptide.
After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 1,000.
Personal Communication, Toru Miki,CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ECT2
Entrez GeneID
1894Protein Accession#
NP_060568;Q9H8V3Gene Name
ECT2
Gene Alias
FLJ10461, MGC138291
Gene Description
epithelial cell transforming sequence 2 oncogene
Omim ID
600586Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq
Other Designations
epithelial cell transforming sequence 2 oncogene protein
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Interactome
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Disease
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Publication Reference
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MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis.
Zhao WM, Fang G.
PNAS 2005 Sep; 102(37):13158.
Application：IF, WB-Ce, IP-WB, Human, HeLa S3 cells.
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Inhibition of cyclin-dependent kinase 1 induces cytokinesis without chromosome segregation in an ECT2 and MgcRacGAP-dependent manner.
Niiya F, Xie X, Lee KS, Inoue H, Miki T.
The Journal of Biological Chemistry 2005 Oct; 280(43):36502.
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The tandem BRCT domains of Ect2 are required for both negative and positive regulation of Ect2 in cytokinesis.
Kim JE, Billadeau DD, Chen J.
The Journal of Biological Chemistry 2005 Feb; 280(7):5733.
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MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis.
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