Gja5 polyclonal antibody
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Specification
Product Description
Sheep polyclonal antibody raised against synthetic peptide of Gja5.
Immunogen
A synthetic peptide (conjugated diphtheria toxoid) corresponding to amino acids 253-269 of rat Gja5.
Sequence
SLVQGLTPPPDFNQCLK
Host
Sheep
Theoretical MW (kDa)
38, 40
Reactivity
Rat
Specificity
Western blotting of cell membarnes of COS cells transiently transfected with cDNA encoding Connexin-40 shows two bands of molecular weight 38 and 40 KDa which are abolished by preincubation with the immunising peptide. No such bands are detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37, Connexin-43 or Connexin-45.
Form
Lyophilized
Recommend Usage
Immunofluorescence (1:10000-1:50000)
Western Blot (1:10000-1:50000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
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Gene Info — Gja5
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