PKMYT1 (phospho T495) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) is used in Western blot to detect Phospho-PKMYT1-T495 in HL-60 (left) and SK-BR-3 (right) cell lysates
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Dot Blot (Peptide)
Dot blot analysis of PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PKMYT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T495 of human PKMYT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Dot Blot (1:500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) is used in Western blot to detect Phospho-PKMYT1-T495 in HL-60 (left) and SK-BR-3 (right) cell lysatesImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Dot Blot (Peptide)
Dot blot analysis of PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — PKMYT1
Entrez GeneID
9088Protein Accession#
NP_004194;Q99640Gene Name
PKMYT1
Gene Alias
DKFZp547K1610, FLJ20093, MYT1
Gene Description
protein kinase, membrane associated tyrosine/threonine 1
Omim ID
602474Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase|protein kinase Myt1
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Interactome
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Pathway
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Publication Reference
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Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
Dai X, Yamasaki K, Yang L, Sayama K, Shirakata Y, Tokumara S, Yahata Y, Tohyama M, Hashimoto K.
The Journal of Investigative Dermatology 2004 Jun; 122(6):1356.
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Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
Nakajima H, Toyoshima-Morimoto F, Taniguchi E, Nishida E.
The Journal of Biological Chemistry 2003 Jul; 278(28):25277.
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The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
Passer BJ, Nancy-Portebois V, Amzallag N, Prieur S, Cans C, Roborel de Climens A, Fiucci G, Bouvard V, Tuynder M, Susini L, Morchoisne S, Crible V, Lespagnol A, Dausset J, Oren M, Amson R, Telerman A.
PNAS 2003 Mar; 100(5):2284.
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Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
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