SIRT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SIRT1 polyclonal antibody (Cat # PAB0004). Western blot analysis of SIRT1 in human pancreas.
(SIRT is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)
Western Blot (Cell lysate)
SIRT1 polyclonal antibody (Cat # PAB0004). Western blot analysis of SIRT1 in Jurkat.
(SIRT is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)
Immunofluorescence
Subcellular localization of SIRT1 on 293T cell.
Immunoprecipitation
Measurement of 293T cell endogenous SIRT1 activity in an immunoprecipitate using SIRT1 polyclonal antibody (Cat # PAB0004) by means of SIRT1/Sir2 Deacetylase Fluorometric (Human) Assay Kit (Cat # KA0105).
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Specification
Product Description
Rabbit polyclonal antibody raised against a recombinant full length SIRT.
Immunogen
Recombinant protein corresponding to full length SIRT.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
SIRT1 Antibody detects endogenous levels of SIRT1 protein. The antibody does not cross-react with type I and II HDAC proteins.
Recommend Usage
Immunoprecipitation (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM phosphate buffer, 150 mM NaCl, pH 7.2 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
SIRT1 polyclonal antibody (Cat # PAB0004). Western blot analysis of SIRT1 in human pancreas.
(SIRT is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)Western Blot (Cell lysate)
SIRT1 polyclonal antibody (Cat # PAB0004). Western blot analysis of SIRT1 in Jurkat.
(SIRT is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)Immunofluorescence
Subcellular localization of SIRT1 on 293T cell.Immunoprecipitation
Measurement of 293T cell endogenous SIRT1 activity in an immunoprecipitate using SIRT1 polyclonal antibody (Cat # PAB0004) by means of SIRT1/Sir2 Deacetylase Fluorometric (Human) Assay Kit (Cat # KA0105). -
Gene Info — SIRT1
Entrez GeneID
23411GeneBank Accession#
NM_012238Protein Accession#
NP_036370Gene Name
SIRT1
Gene Alias
SIR2L1
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Omim ID
604479Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000019691|OTTHUMP00000060745|SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
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Interactome
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Disease
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Publication Reference
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Edible Bird's Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increaing Bone Estrogen-Receptor Expression.
Zhi-Ping Hou, Shi-Ying Tang, Hai-Ru Ji, Pei-Yuan He, Yu-Hong Li, Xian-Ling Dong, Meng-Nan Du, Ismail Maznah, Wen-Jing He.
Chinese Journal of Integrative Medicine 2021 Apr; 27(4):280.
Application：IHC-P, Rat, Rat femurs.
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Edible Bird's Nest Prevents Menopause-Related Memory and Cognitive Decline in Rats via Increased Hippocampal Sirtuin-1 Expression.
Hou Z, He P, Imam MU, Qi J, Tang S, Song C, Ismail M.
Oxidative Medicine and Cellular Longevity 2017 Sep; 2017:7205082.
Application：IHC-P, Rat, Rat brain.
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Edible Bird's Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increaing Bone Estrogen-Receptor Expression.
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