HHLA2 (Human) Recombinant Protein
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Specification
Product Description
Human HHLA2 (Q9UM44-1, Ile23-Asn344) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
Ile23-Asn344
Host
Human
Theoretical MW (kDa)
63.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 94% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 52.7 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD28H, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 52.7 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200). -
Gene Info — HHLA2
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