CD209 (Human) Recombinant Protein
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Specification
Product Description
Human CD209 (Q9NNX6-1, Gln59-Ala404) partial recombinant protein with His tag at N-Terminus expressed in HEK293 cells.
Sequence
Gln59-Ala404
Host
Human
Theoretical MW (kDa)
40.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD209 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD209 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). -
Gene Info — CD209
Entrez GeneID
30835Protein Accession#
Q9NNX6-1Gene Name
CD209
Gene Alias
CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, MGC129965
Gene Description
CD209 molecule
Gene Ontology
HyperlinkGene Summary
This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants
Other Designations
C-type lectin domain family 4, member L|CD209 antigen|Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin
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Interactome
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Disease
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