PTPRC (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD45, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23 ug/mL determined by ELISA.
SEC-HPLC
The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human PTPRC (P08575-3, Gln26-Lys577) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln26-Lys577
Host
Human
Theoretical MW (kDa)
61.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.23 ug/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD45, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 0.23 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — PTPRC
Entrez GeneID
5788Protein Accession#
P08575-3Gene Name
PTPRC
Gene Alias
B220, CD45, CD45R, GP180, LCA, LY5, T200
Gene Description
protein tyrosine phosphatase, receptor type, C
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptide
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Interactome
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Pathway
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Disease
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