LILRB2 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human LILRB2, hFc Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 14.6 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human LILRB2 (Q8N423-1, Gln22-His458) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln22-His458
Host
Human
Theoretical MW (kDa)
74.0999999999998
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 14.6 ng/mL, messured by ELISA at 1 ug/mL. The affinity constant of 9.20 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human LILRB2, hFc Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 14.6 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200). -
Gene Info — LILRB2
Entrez GeneID
10288Protein Accession#
Q8N423-1Gene Name
LILRB2
Gene Alias
CD85D, ILT4, LILRA6, LIR-2, LIR2, MIR-10, MIR10
Gene Description
leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
Omim ID
604815Gene Ontology
HyperlinkGene Summary
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
Ig-like transcript 4|OTTHUMP00000067358|OTTHUMP00000067463|eukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6|immunoglobulin-like transcript 4|leukocyte immunoglobulin-like receptor 2|leukocyte immunoglobulin-like receptor subfa
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