APLN (Human) Recombinant Protein

Catalog # P9933

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Size:100 ug
Price: USD $ 479.00
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  • +1-909-264-1399
    +1-909-992-0619
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  • +1-909-992-3401
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

Immobilized Human APLN, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 22.2 ng/mL determined by ELISA.

Tris-Bis PAGE
QC Test

Tris-Bis PAGE

Human APLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

  • Specification

    Product Description

    Human APLN (Q9ULZ1, Gly23-Phe77) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

    Sequence

    Gly23-Phe77

    Host

    Human

    Theoretical MW (kDa)

    32.9

    Form

    Lyophilized

    Preparation Method

    Mammalian cell (HEK293) expression system

    Purity

    > 95% as determined by Tris-Bis PAGE

    Endotoxin Level

    < 1 EU per 1 ug of protein (determined by LAL method)

    Activity

    The EC50 was 22.2 ng/mL, messured by ELISA at 0.5 ug/mL.

    Quality Control Testing

    Tris-Bis PAGE

    Tris-Bis PAGE

    Human APLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

    Recommend Usage

    Biological Activity
    ELISA
    SDS-PAGE
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from sterile distilled Water is > 100 ug/mL

    Storage Instruction

    Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Result of bioactivity analysis

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Immobilized Human APLN, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 22.2 ng/mL determined by ELISA.

    Functional Study

    SDS-PAGE

  • Gene Info — APLN

    Entrez GeneID

    8862

    Protein Accession#

    Q9ULZ1

    Gene Name

    APLN

    Gene Alias

    XNPEP2

    Gene Description

    apelin

    Omim ID

    300297

    Gene Ontology

    Hyperlink

    Gene Summary

    Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons (De Mota et al., 2004) [PubMed 15231996].[supplied by OMIM

    Other Designations

    apelin, AGTRL1 ligand|peptide ligand for APJ receptor

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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