APLN (Human) Recombinant Protein
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Specification
Product Description
Human APLN (Q9ULZ1, Gly23-Phe77) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly23-Phe77
Host
Human
Theoretical MW (kDa)
32.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 22.2 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human APLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human APLN, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 22.2 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — APLN
Entrez GeneID
8862Protein Accession#
Q9ULZ1Gene Name
APLN
Gene Alias
XNPEP2
Gene Description
apelin
Omim ID
300297Gene Ontology
HyperlinkGene Summary
Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons (De Mota et al., 2004) [PubMed 15231996].[supplied by OMIM
Other Designations
apelin, AGTRL1 ligand|peptide ligand for APJ receptor
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