FGFR2 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human FGFR2 alpha (IIIb), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).
SEC-HPLC
The purity of-Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
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Specification
Product Description
Human FGFR2 (P21802-3, Arg22-Glu378) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Arg22-Glu378
Host
Human
Theoretical MW (kDa)
40.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 6.1 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of-Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human FGFR2 alpha (IIIb), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).Functional Study
SDS-PAGE
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Gene Info — FGFR2
Entrez GeneID
2263Protein Accession#
P21802-3Gene Name
FGFR2
Gene Alias
BEK, BFR-1, CD332, CEK3, CFD1, ECT1, FLJ98662, JWS, K-SAM, KGFR, TK14, TK25
Gene Description
fibroblast growth factor receptor 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq
Other Designations
BEK fibroblast growth factor receptor|FGF receptor|OTTHUMP00000020621|OTTHUMP00000020629|bacteria-expressed kinase|hydroxyaryl-protein kinase|keratinocyte growth factor receptor|protein tyrosine kinase, receptor like 14|soluble FGFR4 variant 4
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Interactome
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Pathway
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Disease
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