Products

Product Description

Human FSTL3 (O95633-1, Met27-Val263) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Sequence

Met27-Val263

Host

Human

Theoretical MW (kDa)

26.1

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Activity

The EC₅₀ was 18.4 ng/mL, messured by ELISA at 0.5 ug/mL. Measured by the ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.

Quality Control Testing

Tris-Bis PAGE

Tris-Bis PAGE

Human FSTL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
Cell proliferation assay
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from sterile distilled Water is > 100 ug/mL

Storage Instruction

Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis