PRLR (Human) Recombinant Protein
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Specification
Product Description
Human PRLR (P16471-1, Gln25-Asp234) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln25-Asp234
Host
Human
Theoretical MW (kDa)
25.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 12.3 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.96 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human PRLR is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human PRLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human PRLR, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 12.3 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human PRLR, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-PRLR Antibody, hFc Tag with an affinity constant of 0.96 nM as determined in SPR assay (Biacore T200). -
Gene Info — PRLR
Entrez GeneID
5618Protein Accession#
P16471-1Gene Name
PRLR
Gene Alias
hPRLrI
Gene Description
prolactin receptor
Omim ID
176761Gene Ontology
HyperlinkOther Designations
OTTHUMP00000115998|delta 4-SF1b truncated prolactin receptor|delta 4-delta 7/11 truncated prolactin receptor|hPRL receptor|prolactin receptor delta 7/11|secreted prolactin binding protein
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