GDF15 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GFRAL, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2 ng/mL determined by ELISA.
SEC-HPLC
The purity of Biotinylated Human GDF15 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Biotinylated Human GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human GDF15 (Q99988, Ala197-Ile308) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Ala197-Ile308
Host
Human
Theoretical MW (kDa)
37.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 4.2 ng/mL, messured by ELISA at 1 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Biotinylated Human GDF15 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Biotinylated Human GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GFRAL, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — GDF15
Entrez GeneID
9518Protein Accession#
Q99988Gene Name
GDF15
Gene Alias
GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Gene Description
growth differentiation factor 15
Omim ID
605312Gene Ontology
HyperlinkGene Summary
Bone morphogenetic proteins (e.g., BMP5; MIM 112265) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM
Other Designations
NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|PTGF-beta|prostate differentiation factor
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Interactome
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Disease
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