IL13 (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8 ng/mL determined by ELISA.
Surface Plasmon Resonance
Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human IL13 (AAK53823, Gly21-Asn132) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Gly21-Asn132
Host
Human
Theoretical MW (kDa)
39.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 15.8 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.399 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of-Human IL-13 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-13, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 uM as determined in SPR assay (Biacore T200). -
Gene Info — IL13
Entrez GeneID
3596Protein Accession#
AAK53823Gene Name
IL13
Gene Alias
ALRH, BHR1, IL-13, MGC116786, MGC116788, MGC116789, P600
Gene Description
interleukin 13
Gene Ontology
HyperlinkGene Summary
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq
Other Designations
-
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com