Products

Product Description

Human FAP (Q12884-1, Leu26-Asp760) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Sequence

Leu26-Asp760

Host

Human

Theoretical MW (kDa)

112.3

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Activity

The EC₅₀ was 11.1 ng/mL, messured by ELISA at 2 ug/mL. Measured by the ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000-pmol/min/ug (QC Test).

Quality Control Testing

SEC-HPLC and Tris-Bis PAGE

SEC-HPLC

The purity of Human FAP is greater than 90% as determined by SEC-HPLC.

Tris-Bis PAGE

Human FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from sterile distilled Water is > 100 ug/mL

Storage Instruction

Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis