IL5RA (Human) Recombinant Protein
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Specification
Product Description
Human IL5RA (Q01344-1, Asp21-Glu335) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Asp21-Glu335
Host
Human
Theoretical MW (kDa)
62.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 6.9 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-5R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-5, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — IL5RA
Entrez GeneID
3568Protein Accession#
Q01344-1Gene Name
IL5RA
Gene Alias
CD125, CDw125, HSIL5R3, IL5R, MGC26560
Gene Description
interleukin 5 receptor, alpha
Omim ID
147851Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq
Other Designations
CD125 antigen|OTTHUMP00000115257|OTTHUMP00000115373|interleukin 5 receptor type 3|interleukin-5 receptor alpha chain|interleukin-5 receptor alpha subunit
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