PGF (Human) Recombinant Protein
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Specification
Product Description
Human PGF (P49763-1, Leu19-Arg221) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Leu19-Arg221
Host
Human
Theoretical MW (kDa)
48.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 12.8 ng/mL, messured by ELISA at 0.5 ug/mL.
Isotype
PGF (Human) Recombinant Protein
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human PGF is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human PGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human PGF, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — PGF
Entrez GeneID
5228Protein Accession#
P49763-1Gene Name
PGF
Gene Alias
D12S1900, PGFL, PLGF, PlGF-2, SHGC-10760
Gene Description
placental growth factor
Omim ID
601121Gene Ontology
HyperlinkGene Summary
vascular endothelial growth factor-related protein|placental growth factor-like
Other Designations
placenta growth factor|placental growth factor, vascular endothelial growth factor-related protein|placental growth factor-like
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