BST1 (Human) Recombinant Protein
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Specification
Product Description
Human BST1 (Q10588-1, Gly29-Ala293) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly29-Ala293
Host
Human
Theoretical MW (kDa)
31.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.4 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human BST1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human BST1, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 8.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — BST1
Entrez GeneID
683Protein Accession#
Q10588-1Gene Name
BST1
Gene Alias
CD157
Gene Description
bone marrow stromal cell antigen 1
Omim ID
600387Gene Ontology
HyperlinkGene Summary
Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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