EPHB2 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human EPHB2, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human EPHB2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human EPHB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human EPHB2 (P29323-1, Val19-Leu543) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Val19-Leu543
Host
Human
Theoretical MW (kDa)
59.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.0 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human EPHB2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human EPHB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human EPHB2, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — EPHB2
Entrez GeneID
2048Protein Accession#
P29323-1Gene Name
EPHB2
Gene Alias
CAPB, DRT, EPHT3, ERK, Hek5, MGC87492, PCBC, Tyro5
Gene Description
EPH receptor B2
Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
OTTHUMP00000002914|OTTHUMP00000002916|developmentally-regulated eph-related tyrosine kinase|elk-related tyrosine kinase|eph tyrosine kinase 3|ephrin receptor EphB2|prostate cancer-brain cancer susceptibility
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Interactome
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Pathway
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Disease
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