TNFRSF18 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GITR Ligand Trimer, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 0.44 ug/mL determined by ELISA.
SEC-HPLC
The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human TNFRSF18 (Q9Y5U5-1, Gln26-Glu161) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln26-Glu161
Host
Human
Theoretical MW (kDa)
41.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.44 ug/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human GITR is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GITR Ligand Trimer, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 0.44 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — TNFRSF18
Entrez GeneID
8784Protein Accession#
Q9Y5U5-1Gene Name
TNFRSF18
Gene Alias
AITR, GITR, GITR-D
Gene Description
tumor necrosis factor receptor superfamily, member 18
Omim ID
603905Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000001519|OTTHUMP00000001520|OTTHUMP00000001521|TNF receptor superfamily activation-inducible protein|activation-inducible TNFR family receptor|glucocorticoid-induced TNFR-related protein
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Interactome
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Pathway
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Disease
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