BST2 (Human) Recombinant Protein
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Specification
Product Description
Human BST2 (Q10589-1, Asn49-Ser161) partial recombinant protein with His tag at N-Terminus expressed in HEK293 cells.
Sequence
Asn49-Ser161
Host
Human
Theoretical MW (kDa)
14.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 3.6 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human BST2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human BST2, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-BST2 Antibody, hFc Tag with the EC50 of 3.6 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — BST2
Entrez GeneID
684Protein Accession#
Q10589-1Gene Name
BST2
Gene Alias
CD317
Gene Description
bone marrow stromal cell antigen 2
Omim ID
600534Gene Ontology
HyperlinkGene Summary
Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq
Other Designations
tetherin
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Interactome
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Disease
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