CD83 (Human) Recombinant Protein
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Specification
Product Description
Human CD83 (Q01151-1, Thr20-Ala143) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Thr20-Ala143
Host
Human
Theoretical MW (kDa)
40.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 15.3 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD83 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD83 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD83, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 15.3 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CD83
Entrez GeneID
9308Protein Accession#
Q01151-1Gene Name
CD83
Gene Alias
BL11, HB15
Gene Description
CD83 molecule
Omim ID
604534Gene Ontology
HyperlinkGene Summary
immunoglobulin superfamily)|OTTHUMP00000016057|cell-surface glycoprotein
Other Designations
CD83 antigen|CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)|OTTHUMP00000016057|cell-surface glycoprotein
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