KLRC1/KLRD1 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human NKG2A&CD94, hFc Tag at 2 ug/mL (100 uL/Well) on Fc Antibody (2 ug/mL) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human KLRC1/KLRD1 (P26715-1/Q13241-1, Arg100-Leu233/Ser34-Ile179) partial recombinant protein with hFc tag and Flag at N-Terminus expressed in HEK293 cells.
Sequence
Arg100-Leu233;Ser34-Ile179
Host
Human
Theoretical MW (kDa)
41.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 40.8 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human NKG2A&CD94, hFc Tag at 2 ug/mL (100 uL/Well) on Fc Antibody (2 ug/mL) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — KLRC1
Entrez GeneID
3821Protein Accession#
P26715-1;Q13241-1Gene Name
KLRC1
Gene Alias
CD159A, MGC13374, MGC59791, NKG2, NKG2A
Gene Description
killer cell lectin-like receptor subfamily C, member 1
Omim ID
161555Gene Ontology
HyperlinkGene Summary
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. [provided by RefSeq
Other Designations
C-lectin type II protein|NK cell receptor A|NKG2-1/B activating NK receptor|NKG2-A/B type II integral membrane protein|natural killer cell lectin|natural killer group protein 2
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Gene Info — KLRD1
Entrez GeneID
3824Protein Accession#
P26715-1;Q13241-1Gene Name
KLRD1
Gene Alias
CD94
Gene Description
killer cell lectin-like receptor subfamily D, member 1
Omim ID
602894Gene Ontology
HyperlinkGene Summary
Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD94 antigen
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Interactome
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Pathway
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Disease
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