CD300A (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD300A, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 34.4 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human CD300A is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD300A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CD300A (Q9UGN4-1, Leu18-Pro180) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Leu18-Pro180
Host
Human
Theoretical MW (kDa)
18.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 9.4 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD300A is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD300A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD300A, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 34.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CD300A
Entrez GeneID
11314Protein Accession#
Q9UGN4-1Gene Name
CD300A
Gene Alias
CMRF-35-H9, CMRF-35H, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC2, IRp60
Gene Description
CD300a molecule
Omim ID
606790Gene Ontology
HyperlinkGene Summary
The CMRF35 antigen (CMRF35A; MIM 606786), which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes. CMRF35H is recognized by the same antibody and is distinct from CMRF35 (Green et al., 1998 [PubMed 9701027]).[supplied by OMIM
Other Designations
CD300a antigen|CMRF35H leukocyte immunoglobulin-like receptor|leukocyte membrane antigen
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