NTS (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human NTS1, hFc Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.30 ug/mL determined by ELISA.
SEC-HPLC
The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human NTS1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human NTS (P30990, Ser24-Leu163) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Ser24-Leu163
Host
Human
Theoretical MW (kDa)
42.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.30 ug/mL, messured by ELISA at 1 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human NTS1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human NTS1, hFc Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.30 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — NTS
Entrez GeneID
4922Protein Accession#
P30990Gene Name
NTS
Gene Alias
NMN-125, NN, NT, NT/N, NTS1
Gene Description
neurotensin
Omim ID
162650Gene Ontology
HyperlinkGene Summary
This gene encodes a common precursor for two peptides, neuromedin N and neurotensin. Neurotensin is a secreted tridecapeptide, which is widely distributed throughout the central nervous system, and may function as a neurotransmitter or a neuromodulator. It may be involved in dopamine-associated pathophysiological events, in the maintenance of gut structure and function, and in the regulation of fat metabolism. Tissue-specific processing may lead to the formation in some tissues of larger forms of neuromedin N and neurotensin. The large forms may represent more stable peptides that are also biologically active. [provided by RefSeq
Other Designations
neuromedin N|neurotensin/neuromedin N
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Interactome
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Pathway
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Disease
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