CFH (Human) Recombinant Protein
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Specification
Product Description
Human CFH (P08603-1, Ser860-Arg1231) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Ser860-Arg1231
Host
Human
Theoretical MW (kDa)
42.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 14.8 ng/mL, messured by ELISA at 0.2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Factor H is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Factor H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Factor H, His Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 14.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CFH
Entrez GeneID
3075Protein Accession#
P08603-1Gene Name
CFH
Gene Alias
ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, MGC88246
Gene Description
complement factor H
Gene Ontology
HyperlinkGene Summary
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
H factor 1 (complement)|H factor 2 (complement)|OTTHUMP00000033598|age-related maculopathy susceptibility 1|beta-1-H-globulin|beta-1H|complement factor H, isoform b|factor H|factor H-like 1
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Interactome
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