DDR1 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human DDR1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 5.9 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DDR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human DDR1(Q08345-1, Asp21-Ala417) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Asp21-Ala417
Host
Human
Theoretical MW (kDa)
45.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 5.9 ng/mL, messured by ELISA at 0.5 ug/mL. (QC Test). The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200)
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DDR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human DDR1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 5.9 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). -
Gene Info — DDR1
Entrez GeneID
780Protein Accession#
Q08345-1Gene Name
DDR1
Gene Alias
CAK, CD167, DDR, EDDR1, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE
Gene Description
discoidin domain receptor tyrosine kinase 1
Omim ID
600408Gene Ontology
HyperlinkGene Summary
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000029343|OTTHUMP00000029344|OTTHUMP00000029345|OTTHUMP00000029346|OTTHUMP00000029347|PTK3A protein tyrosine kinase 3A|cell adhesion kinase|discoidin domain receptor DDR1d|discoidin domain receptor family, member 1|discoidin receptor tyrosine kin
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Interactome
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Disease
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