IL15RA/IL15 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-15RA & IL-15, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human IL-2 R beta & IL-2 R gamma, hFc Tag with the EC50 of 38.8 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human IL-2 R beta & IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra & IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-15RA&IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL15RA | IL15 (Q13261-1 | P40933-1, Thr27-Pro185) partial recombinant protein with His tag at C-Terminus expressed in HEK294 cells.
Sequence
Ile31-Ser108 | Asn49-Ser162
Host
Human
Theoretical MW (kDa)
23.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-15RA&IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-15RA & IL-15, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human IL-2 R beta & IL-2 R gamma, hFc Tag with the EC50 of 38.8 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-2 R beta & IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra & IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). -
Gene Info — IL15
Entrez GeneID
3600Protein Accession#
Q13261-1(IL-15RA) | P40933-1(IL-15)Gene Name
IL15
Gene Alias
IL-15, MGC9721
Gene Description
interleukin 15
Omim ID
600554Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000164617
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Gene Info — IL15RA
Entrez GeneID
3601Protein Accession#
Q13261-1(IL-15RA) | P40933-1(IL-15)Gene Name
IL15RA
Gene Alias
MGC104179
Gene Description
interleukin 15 receptor, alpha
Omim ID
601070Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine receptor that specifically binds IL15 with high affinity. The receptors of IL15 and IL2 share two subunits, the IL2R beta and IL2R gamma chains. This forms the basis of many overlapping biological activities of IL15 and IL2. The IL2 receptor requires an additional IL2-specific alpha subunit for high affinity IL2 binding. This protein is structurally related to IL2R alpha, but is capable of binding IL15 with high affinity independent of other subunits, which suggests the distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported. The full length sequences of only two variants encoding distinct isoforms are available. [provided by RefSeq
Other Designations
OTTHUMP00000019021|OTTHUMP00000019022
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Interactome
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Pathway
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Disease
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