FLT3LG (Human) Recombinant Protein
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Specification
Product Description
Human FLT3LG (P49771-1, Thr27-Pro185) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Thr27-Pro185
Host
Human
Theoretical MW (kDa)
19.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human FLT3 Ligand, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
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Gene Info — FLT3LG
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Interactome
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Pathway
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Disease
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