IL2RB/IL2RG (Human) Recombinant Protein
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Specification
Product Description
Human IL2RB | IL2RG (P14784 | P31785-1, Ala27-Thr240 | Leu23-Ala262) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Ala27-Thr240 | Leu23-Ala262
Host
Human
Theoretical MW (kDa)
51.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-2 R beta&IL-2 R gamma is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-2 R beta&IL-2 R gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-15RA & IL-15, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human IL-2 R beta & IL-2 R gamma, hFc Tag with the EC50 of 38.8 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-2 R beta & IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). -
Gene Info — IL2RB
Entrez GeneID
3560Protein Accession#
P14784(IL-2 R beta) | P31785-1(IL-2 R gamma)Gene Name
IL2RB
Gene Alias
CD122, P70-75
Gene Description
interleukin 2 receptor, beta
Omim ID
146710Gene Ontology
HyperlinkGene Summary
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. [provided by RefSeq
Other Designations
CD122 antigen|OTTHUMP00000028799|high affinity IL-2 receptor beta subunit|interleukin 2 receptor beta
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Gene Info — IL2RG
Entrez GeneID
3561Protein Accession#
P14784(IL-2 R beta) | P31785-1(IL-2 R gamma)Gene Name
IL2RG
Gene Alias
CD132, IMD4, SCIDX, SCIDX1
Gene Description
interleukin 2 receptor, gamma (severe combined immunodeficiency)
Gene Ontology
HyperlinkGene Summary
The interleukin 2 (IL2) receptor gamma chain (IL2RG), an important signalling component of many interleukin receptors (IL2,IL4,IL7,IL9, and IL15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked gene cause X-linked severe combined immunodeficiency (XSCID). [provided by RefSeq
Other Designations
common cytokine receptor gamma chain|interleukin 2 receptor gamma chain|interleukin 2 receptor, gamma
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Interactome
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Pathway
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Disease
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