FLT3 (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human FLT3 Ligand, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Tris-Bis PAGE
Human FLT3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human FLT3 (P36888-1, Asn27-Asn541) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Asn27-Asn541
Host
Human
Theoretical MW (kDa)
84.90000000000001
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human FLT3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human FLT3 Ligand, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4 ng/mL determined by ELISA (QC Test).Functional Study
Surface Plasmon Resonance
Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200). -
Gene Info — FLT3
Entrez GeneID
2322Protein Accession#
P36888-1Gene Name
FLT3
Gene Alias
CD135, FLK2, STK1
Gene Description
fms-related tyrosine kinase 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq
Other Designations
CD135 antigen|FL cytokine receptor|FLT3 receptor tyrosine kinase|OTTHUMP00000042340|fetal liver kinase 2|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1|tyrosine-protein kinase receptor FLT3
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com