CD96 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD96, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 0.17 ug/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human CD96 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD96 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CD96 (P40200-2, Val22-Met503) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Val22-Met503
Host
Human
Theoretical MW (kDa)
54.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD96 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD96 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD96, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 0.17 ug/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). -
Gene Info — CD96
Entrez GeneID
10225Protein Accession#
P40200-2Gene Name
CD96
Gene Alias
DKFZp667E2122, MGC22596, TACTILE
Gene Description
CD96 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
Other Designations
CD96 antigen|T cell activation, increased late expression|T-cell surface protein tactile
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Interactome
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Disease
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