EGFR (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human ALCAM, hFc Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3 ug/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human EGFR (Q13740-1, Trp28-Ala526) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Trp28-Ala526
Host
Human
Theoretical MW (kDa)
82.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human ALCAM, hFc Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3 ug/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). -
Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
Q13740-1Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com