FCGR3B (Human) Recombinant Protein
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More Files
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Surface Plasmon Resonance
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIIB (NA1) , His Tag with an affinity constant of 0.117 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Fc gamma RIIIB (NA1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human FCGR3B (AAA35881.1, Gly17-Ser200) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly17-Ser200
Host
Human
Theoretical MW (kDa)
22
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Fc gamma RIIIB (NA1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIIB (NA1) , His Tag with an affinity constant of 0.117 uM as determined in SPR assay (Biacore T200). -
Gene Info — FCGR3B
Entrez GeneID
2215Protein Accession#
AAA35881.1Gene Name
FCGR3B
Gene Alias
CD16, CD16b, FCG3, FCGR3
Gene Description
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Omim ID
610665Gene Ontology
HyperlinkGene Summary
low affinity IIIb
Other Designations
Fc fragment of IgG, low affinity IIIb, receptor for (CD16)|Fc-gamma receptor IIIb (CD 16)|low affinity immunoglobulin gamma Fc region receptor III-B
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Interactome
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Pathway
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Disease
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