CTGF (Human) Recombinant Protein
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Specification
Product Description
Human CTGF (Q5M8T4, Gln27-Ala349) partial recombinant protein with hFc tag at N-terminus expressed in HEK293 cells.
Sequence
Gln27-Ala349
Host
Human
Theoretical MW (kDa)
61.77
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CTGF is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CTGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CTGF, hFc Tag at 0.2 ug/mL (100 uL/mL/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CTGF
Entrez GeneID
1490Protein Accession#
Q5M8T4Gene Name
CTGF
Gene Alias
CCN2, HCS24, IGFBP8, MGC102839, NOV2
Gene Description
connective tissue growth factor
Omim ID
121009Gene Ontology
HyperlinkOther Designations
OTTHUMP00000017213|hypertrophic chondrocyte-specific protein 24|insulin-like growth factor-binding protein 8
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