GPC3 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human GPC3 (438-554) VLP at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 24.1 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human GPC3 (438-554) VLP is greater than 95% as determined by SEC-HPLC.
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Specification
Product Description
Human GPC3 (P51654-1, 438 a.a. - 554 a.a.) partial recombinant protein expressed in HEK293 cells.
Sequence
RNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHN
Host
Human
Theoretical MW (kDa)
21.5
Form
Liquid
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 24.1 ng/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC
SEC-HPLC
The purity of Human GPC3 (438-554) VLP is greater than 95% as determined by SEC-HPLC.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS pH 7.4
Storage Instruction
Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GPC3 (438-554) VLP at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 24.1 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — GPC3
Entrez GeneID
2719Protein Accession#
P51654-1Gene Name
GPC3
Gene Alias
DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1
Gene Description
glypican 3
Gene Ontology
HyperlinkGene Summary
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000024058|OTTHUMP00000062492|glypican proteoglycan 3
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