NOTCH3 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Notch 3, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human NOTCH3 (Q9UM47, 1378 a.a. - 1640 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
APEVSEEPRCPRAACQAKRGDQRCDRECNSPGCGWDGGDCSLSVGDPWRQCEALQCWRLFNNSRCDPACSSPACLYDNFDCHAGGRERTCNPVYEKYCADHFADGRCDQGCNTEECGWDGLDCASEVPALLARGVLVLTVLLPPEELLRSSADFLQRLSAILRTSLRFRLDAHGQAMVFPYHRPSPGSEPRARRELAPEVIGSVVMLEIDNRLCLQSPENDHCFPDAQSAADYLGALSAVERLDFPYPLRDVRGEPLEPPEPS
Host
Human
Theoretical MW (kDa)
30.18
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 18.2 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Notch 3, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — NOTCH3
Entrez GeneID
4854Protein Accession#
Q9UM47Gene Name
NOTCH3
Gene Alias
CADASIL, CASIL
Gene Description
Notch homolog 3 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq
Other Designations
Notch homolog 3
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Interactome
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Pathway
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Disease
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